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:: Volume 2, Issue 2 (2-2016) ::
3 2016, 2(2): 45-65 Back to browse issues page
Effect of Aeromonas hydrophila infection with high and normal water temperature on total hemocyte count, total plasma protein and histopathological differences in freshwater crayfish Astacus leptodactylus
A Samnejhad *, M Afsharnasab , S Kakoolaki , A Sepahdari
Abstract:   (1753 Views)

In  this  study,  the  effect  of  water  temperature  on  pathogenesis  of  Aeromonas  hydrophila  in  freshwater  Crayfish  Astacus  leptodactylus  were  investigated.  In  our  study,  The  treatments  Crayfish  have  exposed  to  A  hydrophila  with  concentration  of  3×106 CFUml-1  in  high  water  temperature (25˚C)  and  normal  water  temperature(10-15ċ).  The  untreated  control  groups  in  high  water  temperature (25˚C)  and  normal  water  temperature (10-15 ˚C)  were  disinfected  by  oxytetracyclin  at  concentration  of  100 ppm  for  24  hours.  The  haemolymph  samples  were  withdrawn  for  measuring  of  THC  and  TPP.  For  histopathological  studie,  the  Crayfish  samples  were  fixed  in  Davidson  fixative.  The  results  showed  that  the  value  of  THC  in  control  group1  in  comparing  with  control  group2  and  two  other  treatments    at  48h  after  experiment  time  were  significant  (p<0.05).  Also  the  difference  of  THC  value  between  control  group 2  with  treatment  group2  at  144h  after  experiment  time  was  significant  (p<0.05).  The  finding  of  TPP  value  showed  that  there  was  significant  difference  between  control  group1  and  treatment4  in  last  time  after  experiment  time  (336h)  (p<0.05).  In  infected  Crayfish  to  bacteria  with  high  temperature,  red  and  black  spots  and  also  wound  were  observed.  The  result  of  histopathology  in  heptopancreas,  gill,  heart  and  digestive  tract    showed  hemocyte  aggregation,  necrotized  tissue,  dead  cells  within  pyknosis  and  karyorrehexis  of  nucleus  and  vaculation  of  hepatopancreas.  In  treatment  group1  and  control  group1  no  pathological  changes  of  heart  were  observed.  In  digestive  tract  no  changes  were  appeared  in  treatment1  and  two  others  control  group,  but  aggregation  of  hemocyte  with  pyknosis  of  nuclei  was  revealed  in  concentration  of    3×106  CFUml-1  with  high  temperature.

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Type of Study: Research | Subject: Fish physiology
Received: 2016/01/27 | Accepted: 2016/01/27 | Published: 2016/01/27
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DOI: 10.18331/SFS2016.2.2.4


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Samnejhad A, Afsharnasab M, Kakoolaki S, Sepahdari A. Effect of Aeromonas hydrophila infection with high and normal water temperature on total hemocyte count, total plasma protein and histopathological differences in freshwater crayfish Astacus leptodactylus. 3. 2016; 2 (2) :45-65
URL: http://sifisheriessciences.com/article-1-74-en.html
Volume 2, Issue 2 (2-2016) Back to browse issues page
Survey in Fisheries Sciences Survey in Fisheries Sciences
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