In Vivo therapeutic study of Repigmentation of Depigmented patches in Vitiligo disorder isolated Tyrosinase of Moringa oleifera and other Extracts in Zebra Fish Embryo

Abstract

Zebra fish embryos obtained by mating the Zebra fish Danios were used as a model for preclinical in vivo therapeutic studies. Tyrosinase enzyme isolated from the leaves of Moringa oleifera along with the crude extracts rich in polysterols, flavonoids, phenols and carotenoids analysed qualitatively and quantitatively were utilized in inducing pigmentation in the depigmented patches in the above diseased conditions. The depigmented developing embryos were treated with the Tyrosinase enzyme with a 1ml of concentration, showed a partial repigmentation in them. It was also found that the crude enzyme extract along with the enzyme Tyrosinase obtained also induced repigmentation in the depigmented embryos by involving in development of melanin cells and protecting the cells by enhancing the activity of enzyme Tyrosinase in inducing melanin pigments. It functions by inhibiting the conversion of L-tyrosine of melanin, as a result of this the normal Zebra stripped pattern on the skin was not formed, resulting in the formation of depigmented skin in the developing embryos. Firstly, Vitiligo or Leucoderma like condition is induced in Zebra fish embryos by carrying out depigmentation using kojic acid. The outcome of the above study indicated that the repigmentation induced in the depigmented embryos can be correlated to treatment of Vitiligo and Leucoderma by inducing repigmentation of depigmented patches in the above conditions