Use of Standard Inhibitory Zone Diameter to Compare Piper betel Leaf Extract's Efficacy Against Serratia Colony Over Kanamycin for Potential Antimicrobial Applications

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Sandhya A, Vickram A S

Abstract

Aim: The purpose of this study was to assess the efficacy of Piper betel leaf extract against Serratia Colony versus Kanamycin by using standard inhibitory zone diameter. Materials and Methods: A total of 42 samples were computed by ClinCalc, including 21 samples in group 1 (Piper betel leaf extract) and 21 samples in group 2 (Kanamycin antibiotic isolate), using  a power of 0.8, a confidence interval of 95 percent, and standard deviation of 1, the alpha-threshold was kept at 0.05. The findings of the mean results of the zone of inhibition and antibacterial susceptibility, as well as the standard deviation, standard error mean, and the significance of the study, were determined using SPSS version 26 software.The Serratia inoculum was made by inoculating pure Serratia colonies into 10ml of nutrient broth and incubating them for 24hrs. Standard disc diffusion techniques were used to assess antibiotic sensitivity with varied doses of Piper betel leaf extract and kanamycin discs. During MIC, a 96-well plate had been inoculated with 1000µl of nutrient broth, 10 liters of Serratia, and various concentrations of Piper betel leaf extract.Results:The zone of inhibition was estimated with a vernier caliper from the outer edge of the disc/well to the inhibitory zone's outer perimeter.The zone of inhibition was calculated to a P value of 0.036 alongside the P value of <0.001 for antibacterial susceptibility making it significant.Through the various OD values calculated, antimicrobial sensitivity was estimated. The data were analyzed in order to determine which antibiotic was the most successful. Conclusion: In terms of antimicrobial sensitivity, the antibiotic isolate kanamycin was proven to be highly active than the herbal extract Piper betel towards the Serratia colony.

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