Study of antimicrobial activity of zinc oxide nanoparticles and Phenol extract from Nigella Sativa L.

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Khetam L. Hussain, Nibras Al-ibrahemi, Heba Khlaf Yassin

Abstract

Background: This study investigate the antibacterial activity of phenol extract from Nigella Sativa L and Zno nanoparticles toward staphylococcuse aureas and Escherichia coli. Objective: Detecting the phytochemical of plant by using reagent, separation phenol compound from alcohol extract and studding antibacterial of phenol extract and Znonanoparticl.                                                                                                                                           Patients and methods: the antibacterial test detected by disc diffusion methods at concentration ( 25,50,75 and 100 mg/ml ) for phenol extract , while Zno nanoparticles at concentration (0.2 , 0.3, 0.4 and 0.5 mg/ml). Results: the inhibition zone of phenol extract in concentration 25 mg/ml was 16.11 mm, 15.11 mm for S. aureus, E .coli respectively, while concentration 50 mg/ml in S. aureus, E.coli 17.44mm, 16.12mm respectively, while concentration 75mg/ml in S. aureus 19.22mm, E.coli 18.34 mm , concentration 100 mg/ml in S. aureus 21.12mm, E.coli 20.12 mm. While the inhibition zone of Zno nanoparticles was 1.23 mm, 2.23 mm against S. aureus ,E .coli respectively with concentration0.2 mg/ml, while concentration 0.3mg/ml in S. aureus 1.99mm, E.coli 3.54mm, while concentration 0.4mg/ml in S. aureus 2.76mm, E.coli 4.11 mm , concentration 0.5 mg/ml in S. aureus 3.65, E.coli 4.11 mm. Conclusion: This effect due to the ability of ZnO nanocomposite to disrupt genes for resistance to oxidative stress, and increase the ROS, which contributes to eradication of bacteria by stimulating macrophages and production of cytokines. Also disrupt the bacterial cell wall (positive, negative) by affecting the osmosis of the cell wall, so the cells treated with this compound appear wrinkled and irregular in shape.                                                                               

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