High Performance Thin Layer Chromatography Fingerprinting of Phytosterols from Hydroalcoholic Extract of Holoptelea Integrifolia (Roxb.) Leaves

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Ravindra C. Sutar, Gowtham M, Girish A. Kashid

Abstract

Plant Sterols (PS) (Phytosterols And Phytostanols) Are Bioactive Compounds Of All Vegetable Foods Where Can Be Found As Free Sterol Alcohols And As Conjugates. These Latter Forms Have Been Less Studied, Although They May Have Potential Beneficial Effects, Whereas Some PS Have Several Approved Health Claims. Holoptelea Integrifolia Belongs To The Family Ulmaceae Commonly Called As Indian Elm And Commonly Used In India By The Tribal People For It’s Medicinal Properties. Plants And Plant-Based Products Are The Basis Of Many Of The Modern Pharmaceuticals We Use Today For Various Ailments. Authentication Of Natural Products Can Be Done With Precious Method Like High Performance Thin Layer Chromatography (HPTLC). High Performance Thin Layer Chromatography (HPTLC) Technique Was Utilised To Detect Phytosterols From Hydroalcohol Extract Of Holoptelea Integrifolia. Phytochemical Screening Was Performed And Later HPTLC Studies Were Done. Instrumentation For HPTLC With CAMAG System Having Linomat V Applicator (Switzerland).  Camag TLC Scanner IV With Reflectance Absorbance Mode At 277nm, Equipped With Win CATS Software (1.4.6 Camag), Tungstant Lamp Were Used To Perform Densitometric Scanning. Steroids, Tannins, Alkaloids, Glycosides, Flavonoids, 


Phytosterols Were Found In Holoptelea Integrifolia Hydroalcohol Extract Undergoing Preliminary Phytochemical Analysis. Our Aim Is To Do HPTLC Studies From Hydroalcohol Extract Of Leaves Of Holoptelea Integrifolia. Objective That Were Assessed Are Hydroalcohol Extract Of Leaves Of Holoptelea Integrifolia. HPTLC Finger Printing Of Phytosterols Of Hydroalcohol Extract Of Leaves Revealed Twelve Multivalent Phytochemicals Having Increasing Order Of Rf Values 0.10- 0.93. Rf 0.40 Has 46.10% Concentration.Phytosterols In Hydroalcohol Extract Were Confimed By Preliminary Phytochemical And Rf Values.

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