Molecular detection of some β-lactamase genes among K. pneumonia 258

Abstract

In this cross-sectional investigation, researchers gathered 100 samples from patients at Hilla Teaching Hospital and Marjan Medical City. Using morphological, microscopic, biochemical, and Vitek II system testing, researchers were able to identify 40 distinct strains of K. pneumonia from 100 samples collected from a wide range of infection sites. Standard PCR was performed on the DNA from all of the probable K. pneumonia isolates to amplify the pilv-l gene using the sequences; the findings showed that only 16 (40%) of the 40 K. pneumonia isolates were connected to K. pneumonia258 by sharing the same 320 bp DNA fragment with the allelic ladder. Some virulence genes were detected in 16 K. pneumoniae258 isolates and compared with local K. pneumonia, the results showed that, BlaOXA-48 gene were detected in 13/16(81.2%) were positive to blaOXA-48 gene of K. pneumoniae258 isolates while 8/16(50%) K. pneumoniae isolates were found at (428bp). However, blaTEM gene were detected in 13/16(81.2%) were positive to blaTEM gene of K. pneumoniae258 isolates while 9/16(56.2%) K. pneumoniae isolates were found at (1080bp). blaSHV gene were detected in 12/16(75%) were positive to blaSHV gene of K. pneumoniae258 isolates while 10/16(62.5%) K. pneumoniae isolates were found at (930bp), blaCTX-M gene were detected in 15/16(93.7%) were positive to blaCTX-M gene of K. pneumoniae258 isolates while 8/16(50%) K. pneumoniae isolates were found at (585bp).