Evaluate phage single and cocktail as therapy against bedsores infection causes by some MDR pathogenic bacteria

Abstract

Background: Multiple drug resistance (MDR) and extensive drug resistance (XDR) strains, in addition to the fact that there is no overarching strategy for the discovery and manufacturing of novel, All of these variables contribute to the development of antibiotic-resistant bacteria and the illnesses they cause. The need for alternative therapies has become more clear as a result of antibiotics. Methods In this investigation, certain phages that influence XDR Pseudomonas aeruginosa strains were identified and PDR Acinetobacter baumanii were separated out from sewage water at a hospital, and their lab properties as well as their lysis effect on 10 MDR,XDR strains of P. aeruginosa and 4 PDRand XDR A. baumanii strains were investigated .

Results: Virulent phages towards bacterial isolates were isolated from wastewater of hospitals and oil refinery from Karbala and identified by spot lysis method,and double layer agar plaque assay .Four phages were isolated, one phage against XDR P.aeruginosa and three phages against PDR A.baumanii.All the phages that isolated and characterized by Transmission electron microscope the phages belonged to the order of those with tails (Caudovirales) and family of Siphoviridae and Podoviridae. Each phage is Extensively resistant - Pseudomonas aeruginosa were found to lyse P.aeruginosa 20- 24%.While the same species phage cocktail that represents (P-AP3) and mixed with species(P-AA5) the phage cocktail two or three of phages that represents all phages were found to lyse, 70-80%. Conclusion: The findings of this study indicate that these phages have a significant potential for therapeutic use and prophylaxis of infections caused by this bacterium.