Physicochemical characterization, cytotoxicity and anticancer activity of L-glutaminase from marine Streptomyces luteogriseus

Abstract

The biological activities of L-glutaminase from bacterial sources have been a subject of significant interest. In this study, we isolated Streptomyces luteogriseus from marine sediment and identified it as a potential producer of L-glutaminase through molecular identification using 16S rRNA sequencing. On SDS-PAGE analysis, the isolated L-glutaminase migrated at a molecular weight of 50 KDa. We optimized L-glutaminase production by investigating various factors such as pH, temperature, metal ions, additives and substrate specificity to enhance production efficiency. Our findings suggest that Streptomyces luteogriseus from marine sediment has the potential to produce L-glutaminase, making it a promising candidate for future research in this field.