In Vitro Cytotoxicity Studies on Hydro-Alcoholic Aerial Parts Extract of Sesamum Prostratum Retz.

Abstract

One of the deadliest diseases threatening the human being is Cancer. Cancer is the second leading cause of death globally and among which Breast cancer is second leading cause of death in women. Breast cancer may be provoked due to several physical, chemical and environmental factors. Moreover, genetic alterations that are inherited via generation may be a reason for the occurrence of cancer. If breast cancer is not treated during initial stages, it may spread to nearby organs, a process called metastasis, through the blood or lymph system. The rising burden of cancer worldwide calls for an alternative treatment solution. Medicinal plants serves as an important tool for the treatment of various diseases and disorders. Herbal medicine provides a very feasible alternative to western medicine against cancer. This could be an alternative to other treatment methods, such as they cause minimal side effects when compared to chemo-drugs. In the present study, in vitro cytotoxic effect of hydro-alcoholic extract of Sesamum prostratum Retz. Aerial parts were evaluated against normal and cancer cell lines at five different concentrations such as 5µg/ml, 25 µg/ml, 50 µg/ml, 75 µg/ml and 100 µg/ml. The percentage viability was found to be increasing with increasing concentration of test compound against the normal (L929) and cancer (MCF-7) cell lines. The extract was found to be cytotoxic against normal and cancer cell lines. The study indicates that, aerial parts can be better crude drug of choice against Human Breast cancer. The results revealed that, hydro-alcoholic extract Sesamum prostratum Retz. Have better IC₅₀ value 63 µg/ml against MCF-7 (Human Breast Cancer) cell line than the normal cell line IC₅₀ 30 µg/ml.