In vitro evaluating total flavonoids, DPPH radical scavenging activity and cytotoxic effects of sage (Salvia officinalis) methanolic extract

Abstract

This study aimed to evaluate the cytotoxic effects of sage methanolic extract different cancer cell lines (prostate PC3, liver HepG2, lung A549 and breast MCF7 cancer cell) after estimation total flavonoid and  DPPH free radical scavenging activity. Salvia officinalis L., the regular sage is the most agent species inside the family of Labiatae. Healing properties of sage are especially perceived since most punctual occasions and its utilization as a tonic, stimulant, carminative, disinfectant and antihydrotic. Results indicated that total flavonoids in sage was 175.73 ± 5.78 µg/ml and DPPH scavenging activity was concentration dependant ranged from32.1± 1.4% to 72.8 ± 4.2 % for 0.0620 to 0.500 mg/ml respectively). Sage possessed the most potent toxicity on lung cancer cell in which non-viable cell reached to 43.04±3.32% for 1 mg\ml concentration while little effect observed on breast cancer cell line.