The Phenotypic Characterization of Symptomatic and Asymptomatic Blastocystis hominis Subtypes Isolated from Irritable Bowel Syndrome Patients in Diwanyiah City of Iraq

Abstract

The phenotypic characterization of protein profiles differences between the pathogenic and non-pathogenic Blastocystis hominis and this differences explained the phenomenon of intra subtype variation in pathogenicity. The present study was aimed to investigate phenotypic characteristics of Blastocystis hominis based protein profiles by SDS PAGE analysis was used to discriminate between pathogenic potential symptomatic and asymptomatic Blastocystis hominis subtypes isolates. The SDS-PAGE protein profiles analysis results of symptomatic and asymptomatic Blastocystis hominis positive subtypes isolates were showed the presence high variable numbers of both high and low weight (KDa) protein bands in Blastocystis hominis positive ST3, ST7, and ST1 subtypes. The Blastocystis hominis positive subtype ST3 isolates were showed 19 protein bands in symptomatic patients isolates which ranged from (200KDa-17KDa) and 17 protein bands in asymptomatic patients isolates which ranged from (135KDa-17KDa). The Blastocystis hominis positive subtype ST7 isolates were showed 13 protein bands in symptomatic patients isolates which ranged from (80KDa-30KDa) and 8 protein bands in asymptomatic patients isolates which ranged from (80KDa-30KDa). The Blastocystis hominis positive subtype ST1 isolates were showed 16 protein bands in symptomatic patients isolates which ranged from (180KDa-30KDa) and 13 protein bands in asymptomatic patients isolates which ranged from (80KDa-30KDa). Statistical analysis of protein profiles revealed significant differences in symptomatic and asymptomatic Blastocystis hominis positive subtypes isolates. In Blastocystis hominis subtype 3 was showed high significant differences at (P value 0.0034) and found (76.42%) in symptomatic isolates and (45.37%) in asymptomatic Blastocystis hominis subtype 3 isolates The Blastocystis hominis subtype 7 was showed non-significant differences at (P value 0.1092) and found (61.54%) in symptomatic isolates and (38.46%) in asymptomatic Blastocystis hominis subtype 7 isolates. The Blastocystis hominis subtype 1 was showed significant differences at (P value 0.0193) and found (66.12%) in symptomatic isolates and (39.06%)in asymptomatic Blastocystis hominis subtype 1 isolates. In conclusion, this work is demonstrating the presence of a significant difference in protein profiles between symptomatic and asymptomatic Blastocystis hominis isolates and The pathogenic potential was related especially into asymptomatic Blastocystis hominis subtype 3 isolates.