Isolation, identification and phosphate solubilization of fungi from soil in Jaipur district of Rajasthan

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Nupur Bhatnagar, Tripti Yadav

Abstract

The soil act as a great reservoir of microorganisms, play different roles in the environment. Fungi is one of kind of microorganisms that have a major role in converting the dead and decaying matter into organic substances which are further used as fertilizers and increase the soil nutrients. The aim of the present study is to isolate and identify fungal species present in different soil samples of Jaipur, Rajasthan. The isolated fungal species were also subjected to phosphate solubilization activity which was calculated by solubilization index (SI) value. A total of ten fungal species including Aspergillus niger, Aspergillus nidulans, Aspergillus flavus, Aspergillus fumigatus, Fusarium oxysporum, Rhodotorula mucilaginosa, Rhizopus stolonifer, Sepedonium chrysospermum, Penicillum expansum, and Cladiosporium sphaerosperimum were isolated from different locations on the basis of morphological and microscopic characterization. The results obtained for phosphate solubilization; it was observed that all the isolated fungal species showed halo zone around the fungal growth with SI value varying from 1.0 to 1.4. Phosphate-solubilizing saprophytic fungi have a potential application in plant nutrition as phosphate is a macronutrient and play a vital role in every aspect of plant growth and development. With the help of phosphate solubilizing fungi present in soil, it can hydrolyze organic and inorganic insoluble phosphorous compound to soluble ones that can easily be assimilated by plant therefore can be used in bio fertilizer industries.

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