Fingerprinting Of Flavonoids from Hydroalcoholic Extract of Holoptelea Integrifolia (Roxb.) Leaves Using Hptlc

Abstract

Background: Holoptelea integrifolia belongs to the family ulmaceae commonly called as               Indian Elm and commonly used in India by the tribal people for it’s medicinal properties. Plants and plant-based products are the basis of many of the modern pharmaceuticals we use today for various ailments. Authentication of natural products can be done with  precious method like High performance thin layer chromatography (HPTLC).

Objective: High Performance Thin Layer Chromatography (HPTLC) technique was utilised to detect flavonoids from hydroalcohol extract of Holoptelea integrifolia.

Methods: Phytochemical  screening  was performed  and later  HPTLC  studies  were  done. Instrumentation for HPTLC with CAMAG system having Linomat V applicator (Switzerland).  Camag TLC scanner IV with reflectance absorbance mode at 277 nm , equipped with Win CATS software (1.4.6 Camag), tungstant lamp were used to perform densitometric scanning.

Results: Steroids, tannins, alkaloids, glycosides, flavonoids, phytosterols were found in Holoptelea integrifolia Hydroalcohol extract undergoing preliminary phytochemical analysis. HPTLC finger printing of Flavonoids of hydroalcohol extract of leaves revealed twelve multivalent phytochemicals having increasing orderof Rf  values 0.05- 0.90 Rf 0.90 has 16.17% concentration

Conclusions:  Flavonoids in hydroalcohol extract were confimed by preliminary phytochemical and Rf Values