Molecular and immunological detection of Toxoplasma gondii and Cytomegalovirus in Aborted Women with Covid -19

Abstract

Toxoplasma gondii and Cytomegalovirus infections are the most common causes of congenital infections worldwide in addition the coronavirus disease is a serious public health emergency and a previous report said pregnant women usually developed a mild form of COVID-19 despite a few cases for acute form with maternal morbidity and mortality . So, this study aimed to molecular and immunological detection of Toxoplasma gondii and Cytomegalovirus  in aborted Women with Covid -19.   This study involved 719 samples taken from women with recurrent pregnancy loss who attending the Maternity and Children Hospital in addition to some medical laboratories in Al-Diwaniyah Governorate during the period from February 2021 to September 2022, their ages ranged between (18-47) years. For the purpose of diagnosing infection with Human cytomegalovirus and Toxoplasma gondii, where IgG and IgM were evaluated for both using immunodiagnosis by rabid test and ELISA, and then the results were confirmed using RT-PCR. Also, their was relationship to previous infection with Covid-19 in a studied aborted women. The results obtained by rabid test and ELISA were 45 for HCMV, 40 for T. gondii, and 11 for HCMV and T. gondii together in the same patient, and when confirming the results of the diagnosis by RT-PCR, it showed less positive results, amounting to 28 for HCMV and 22 for T. gondii. Also, it was found that there was a relationship between previous infection with Covid-19 and the activation of latent chronic infection of HCMV and T. gondii in aborted women.