Biosynthesis of Silver Nanoparticles from Klebsiella pneumoniae and analysis of its Haemato-Toxic and Antibacterial Properties.

Main Article Content

Kunal Madhav, Archana Pandita

Abstract

 


Biogenic silver nanoparticles are in great demand for their multipotential role including the elimination of drug resistant pathogens. In the present study we have biosynthesized the silver nanoparticles (BNPs) from the bacteria Klebsiella pneumoniae which was isolated from the soil sample. These nanoparticles were analyzed for their morphological characteristics and were also evaluated for its antimicrobial activities against multiple drug resistant pyogenic pathogens. Morphological and biochemical characterizations revealed these pathogens to be Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus, among which P. aeruginosa was also found to be an ESBL producer, while others were multi drug resistant. The BNPs were also evaluated for their cytotoxic evaluation on human erythrocytes to characterize the haemo-compatibility nature.  Based on the experiments conducted it was concluded that these silver nanoparticles had a strong antibacterial activity against S. aureus amongst all the pyogenic pathogens, while moderately equivalent activities were displayed against P. aeruginosa and E. coli. Also, these nanoparticles were evaluated for their cytotoxic activities on the human Red Blood Cells on varying concentrations displaying the significantly less haemato-toxic activities. Based on these activities it was concluded that these biosynthesized silver nanoparticles can safely be used as an alternate to the antimicrobial therapy and also for other biomedical applications.


 

Article Details

Section
Articles
Author Biography

Kunal Madhav, Archana Pandita