PCR determination of some virulence gene among Enterobacter cloacae isolated from gall-bladder infections

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Kahlaa Muhammed Abbas Al-Mulla, Abbas Shaker Al-Muhanna

Abstract

Background: Enterobacter cloacae is gram negative rod, widely spread in nature, it is found in soil, sewage, and human gastrointestinal tract, it is one of the common opportunistic pathogens in hospitals environments, which leads to various infections, such as septicemia, pneumonia, urinary tract infections, and postsurgical peritonitis hospital-acquired infections.


Introduction: Acute cholecystitis (AC) is one of the most common surgical diseases. Bacterial infection accounts for 50% to 85% of the disease’s onset, since there is a close relationship between the biliary system and the gut, thus, the study aimed to investigated the role of bacteria in causing gallbladder infections.


Methodology: 174 gallbladder swabs were collected from patient suffering from gall-bladder infection (after cholecystectomy) , after the specimens were cultured on suitable media, the identification of bacterial isolates was carried out using biochemical test and Vitek-2 system, PCR amplification technique were used to investigation the predominance, genes Rpos and papC genes and wcaG among E cloacae isolate


Results: After incubation period the results appear that (47.12%) of specimens were gave bacterial growth and (52.88 %) were appear no growth. E cloacae were identified in (24.39%) of bacterial growth., the results PCR multiplication technique appear that (55%) of isolates carried Rpos genes, while (20%) and (60%) of bacterial isolates were carrying papC and wcaG genes.


Conclusions: Bacteria the major agent of gall-bladder infections and E cloacae was the most common bacteria causing gall-bladder infections.


 


 

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Kahlaa Muhammed Abbas Al-Mulla, Abbas Shaker Al-Muhanna