Determination of Genetic Relationship Using RAPD in Some Species of TRIGONELLA L. from the Fabaceae Family in Central and Northern Iraq

Abstract

The current research included the study of six wild plant species belonging to the genus Trigonella L. of the legume family in central and northern Iraq, and they have medical and economic importance, and because these species are closely related phenotypically and even from an anatomical point of view, the aim of the study is to use the genetic aspect to distinguish between species taxonomically, it was shown through the study The current genetic distance between species using the RAPD marker, as ten primers were used. It varied in showing the number of bands, their location and size, as it was observed that the bundles were absent in some species and their appearance in other types using the same apparent, which indicated the existence of genetic variation between species, as the total number of bands reached 381 bands, and the number of polymorphic bands was 243, and the study recorded the emergence of 138 monomorphic bands While the number of unique bands reached 32 bands distributed over species and different molecular sizes, the study also showed the percentage of polymorphism as it reached 100%, while the discriminating ability reached 16.460 % , It was found through the dendritic diagram that the shortest genetic distance was between the two species T.astroites and T.elliptica, which amounted to 0.51856, while the longest genetic distance was recorded between the two species T.monspliaca and T.stellata and was 0.68599, and this discrepancy is attributed to the occurrence of adaptation to living in the environments in addition to Due to the occurrence of genetic mutations and modifications between species, therefore, the use of the RAPD marker distinguishes between species genetically and the genetic dimension between them.