Isolation and identification of Streptococcus pneumoniae in children in the city of Mosul, and use of an innovative medium for preserving and transporting it

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Saud Ameer Saadullah, Sundus Jassim Mohamad, Dr. Firas Shawqi Algburi

Abstract

 


The study included investigating Streptococcus pneumoniae infection among children in the city of Mosul, investigating the ability of pneumococci to secrete the Hyaluronate lyase, study the kinetics of this enzyme and the investigating of the inhibitory activity of some chemicals towards the activity of the enzyme. The study included (200) cases of children suspected of having Pneumococci distributed between (145) males and (55) females from different hospitals in the city of Mosul for the duration of (November 2021 until March 2022). S.pneumoniae was isolated and diagnosed by culture on brain heart infusion broth, Mueller-hinton agar and blood based agar medium. Then the isolates were purified and diagnosed according to phenotypic characteristics on the culture media, hemolytic phenotype, sensitivity to the antibiotic optochin, solubility in bile salts, agglutination test for pneumococcal antibodies and biochemical tests confirmed diagnosis by using VITEK 2 system. The study showed that out of the total of the suspects (47) cases were confirmed to be infected with pneumococci as resemble (23.5%). The samples taken from them varied and included (61.71% nasopharyngeal swabs, 25.53% acute otitis media, 2.12% cerebrospinal fluid, 0% blood and 10.64% sputum). The results of the antibiotic sensitivity examination of the isolates showed that they were (100% are sensitive to the levofloxacin, meropenem and vancomycin), (100% are resistant to ampicillin, cephalexin and azithromycin). (87.23%) are sensitive to ceftriaxone, (82.98%) are sensitive to chloramphenicol, As for the trimethoprim-sulphamethoxazole, the results showed that (19.14%) were sensitive, tetracycline (19.14%), and (19.51%) are sensitive to clindamycin, penicillin sensitivity showed (65.95%), while augmentin was (93.61%) resistant.


 

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Saud Ameer Saadullah, Sundus Jassim Mohamad, Dr. Firas Shawqi Algburi