Evaluation of the protective action of officinarum extract against bisphenol A-induced oxidative stress and DNA damage in male albino rats

Main Article Content

Mahdi Hamzah Khashan, Rasha Abdulameer Jawad, Nasser Merza Hamza

Abstract

Objective: Antioxidants that reduce oxidative stress, lipid peroxidation, and DNA damage, restoring the global antioxidant defense system, can be used to treat male infertility and poor semen quality associated with bisphenol A (BPA) exposure. this work, aimed to investigate the protective action of Alpinia officinarum  (A. officinarum) rhizome extracts against oxidative stress and DNA damages induced by BPA.


Material and methods: Eighteen adult albino rats were used and divided into three groups; control group received 0.2mL of olive oil/rat/day, BPA group received BPA (50 mg/kg) and protected group received A. officinarum extract (400 mg/kg) then BPA (50 mg/kg) after one hour. The various doses were administrated orally for 60 days. 24 hours after the last dose, the animals were weighed and sacrificed, blood samples were collected for the assessment of oxidant/antioxidant markers, while testes and epididymis were weighed after extraction and cleaning, and epididymis sperm were used to estimate the degree of DNA damage.


Results: The weighted values of the testes and epididymis showed a significant decrease in the BPA group compared to the control group, while the decrease of those values was relatively low in the protected group, as it moved away from the BPA group and approached the control group. The data also showed a significant decline from the normal state in the indicators of oxidation/antioxidants due to the treatment with BPA , while that decline receded to a large degree in the group protected with A. officinarum extract. The results also included a significant increase in the number of sperms that suffered from DNA fragmentation and the amount of that damage in the BPA-treated group, but that damage was much less than in the protected group.


Conclusion: We conclude that A. officinarum extract is effective in reducing the harmful effects of increased oxidative stress and DNA fragmentation that result from exposure to BPA through its anti-free radical formation and scavenging activity.


 


 

Article Details

Section
Articles
Author Biography

Mahdi Hamzah Khashan, Rasha Abdulameer Jawad, Nasser Merza Hamza