The Impact of Interleukin-15 Serum level and Gene Expression in Celiac Disease for Sample of Iraqi Patients\

Abstract

Background and Aims: The inflammatory cytokine IL-15 has a significant role in celiac disease (CD) development. This research aims to determine the impact of serum levels of interleukin-15 and its relationship to gene expression in patients with celiac disease.

Materials and Methods: "Sixty patients with celiac disease and thirty healthy-looking controls had peripheral blood samples obtained. IL-15 serum levels were determined by an enzyme-linked immunosorbent assay (ELISA) technique. Total RNA was extracted, and the mRNA expression levels of the IL-15 gene were investigated by the quantitative real-time polymerase chain reaction (qRT-PCR) method".

Results: There was a statistically significant (p ≤ 0.000) increase in IL-15 serum levels between CD patients and seemingly healthy controls. The IL-15 gene expression was also significantly higher in patients than in the healthy control group (p =≤ 0.000).

Conclusion: The findings indicated that IL-15 could be a valuable and sensible marker for distinguishing celiac disease patients from healthy controls. However, further study is needed to comprehend Interleukin-15's diagnostic value properly.