Identification Multi-Locus Sequence Typing for Salmonellosis Isolates From Effluent Mosul Hospitals Water as an Indicator of Pollution

Abstract

A study was conducted to investigate the presence of Salmonellosis bacteria in hospital effluent water in Mosul, Iraq and assess its potential as an indicator of pollution. The study collected 21 water samples from different hospitals and used Chromogenic agar to isolate Salmonella strains. Multi-Locus Sequence Typing (MLST) was then used to genotype the Salmonella isolates, targeting seven housekeeping genes.

The results of the study showed that thirty seven Salmonella isolates were identified from the twenty one water samples. The use of Chromogenic agar was found to be an effective tool for the detection of Salmonella in hospital effluent water. The MLST analysis of the Salmonella isolates revealed that they belonged to different sequence types, indicating a diverse genetic background. The seven housekeeping genes used in the study have been shown to be effective in discriminating between different Salmonella strains and are commonly used for Salmonella genotyping.

The presence of Salmonella in hospital effluent water is a major concern as it may present a considerable hazard to the general public health. Bacterial is a common cause of foodborne illness and contaminated water can be a potential source of infection The transmission of Salmonella via hospital wastewater can also aid in the development of antibiotic-resistant strains, resulting in increased difficulty in treating infections caused by this pathogen.

The study highlights the importance of proper treatment and disposal of hospital wastewater to prevent the spread of waterborne diseases. The use of Chromogenic agar and MLST can be valuable tools for monitoring the genetic different types of bacterial communities and studying the epidemiology of various bacterial pathogens.