Molecular detection of phzD, phzA and phzG Gene among Pseudomonas aeruginosa Isolated from Burn Infections

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Estabraq Hassan Badder Al-Muhanna, Abbas Shakir Jawaad Al-Muhanna

Abstract

Pseudomonas aeruginosa's ability to produce phenazine poses a severe healthcare issue with a range of effects on patients and the healthcare system. The study's goal was to find out how phenazine affects bacterial burn infections. Because of this, 223 swabs from burn infections were collected and grown on suitable conditions; the initial identification was based on morphological and culture features, biochemical tests, and then identification with the vitek-2 system. Phz D, Phz A, and Phz G genes that code for phenazine synthesis were studied genetically using the PCR technique. All 34 (97%) P. aeruginosa isolates tested positive for the phz D gene, 34 (97%) isolates tested positive for the phz A gene, and 23 (66%) isolates tested positive for the phz G gene.


 


 

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Estabraq Hassan Badder Al-Muhanna, Abbas Shakir Jawaad Al-Muhanna