Evaluation of Antioxidant activity of Camellia sinensis and Vachellia nilotica formulation

Abstract

Aim: To evaluate the antioxidant property of Camellia sinensis (Green Tea) and Vachellia nilotica (Babul) herbal formulation.

Background: In ancient times, green tea and barks of Babul tree were considered by traditional Chinese medicine. In th recent year studies for human suggest that babul the plant which is used in our study might contribute to a reduction in the risk of cardiovascular disease, cancer, hypertension , for controlling body weight, also has antibacterial and antiviral activity, and also has bone mineral density increasing property and anti-fibrotic properties.

Materials and Methods: Green tea and babul  plants extract was produced with 1 gram dried green tea leaves and 1 gram Babul leaf powder dissolved in 100ml distilled water. For 10 mins the prepared extract was boiled , at a temperature of 60-80°C on a heating mantle. Whatman No.1 filter paper was used for filtering the prepared extract. Followed by the green tea and babul herbal formulation was tested to DPPH assay and H2O2 assay to identify the antioxidant activity.

Results: The Green tea and Babul herbal formulation has moderate antioxidant activity at high concentrations (50 μL). However, two standards of both assays have comparatively higher antioxidant activity compared to Green tea and Babul herbal formulation.

Conclusion: This study was concluded that the Green tea and Babul herbal formulations have a moderately efficient antioxidant activity at high concentrations.