Efficacy of Herbal Cellulose Gelatin Foam on 2nd Degree Cutaneous Burn Healing

Abstract

A lot of researchers try many types of therapies for burns in Veterinary medicine in the last decades. This project aims to compare the efficacy of novel therapy, herbal cellulose gelatin foam on 2nd degree cutaneous burn healing. Fifty female white albino rats, who were housed in plastic boxes divided randomly into two groups 25 rats for each. Control group G1 had no treatment, second group G2 treated with herbal cellulose gelatin foam. Sample from cellulose gelatin foam was examined under SEM for the morphology of the foam at 100 µm.

 Under routine surgery with general anesthesia all rats had thermal injuries at the left side of their backs. The thermal injuries were then created using a solid aluminum bar with a diameter of 2 cm in diameter had previously been heated to 100°C in boiling water. The bar is kept in touch with the rat's skin in the dorsal proximal region of the back. Hyperemia, edema, scar tissue, and scab were recorded for morphological exam. The therapies were applied topically once daily for 10 days. At the time of burn and on the appropriate days for the biopsies at 7th, 14th, 21st and 28th day, sterile "swabs" were taken and sent to laboratory for bacterial contamination. Biopsies were collected for histologic evaluation of healing. The morphologic appearance were recorded at 1 day, 7th day, 14th day,21th day, and 28th day, Histologic evaluation showed the superiority of herbal cellulose gelatin foam than control group for 2nd degree burn healing and this result accompanied with the other parameters. Histological changes post burn injury in herbal cellulose gelatin treatment showed complete healing of all layers of skin including epidermis, papillary dermis, reticular dermis , and hypodermis, hair follicles, sweat glands, and sebaceous glands were obvious. The study concluded that herbal cellulose gelatin foam speed up the healing of 2nd degree burn.