Microorganisms of agroindustrial use isolated from the soil of a primary forest of the Pungalá Parish in the Riobamba Canton

Abstract

The objective of this study was to isolate, purify and identify bacteria and yeasts for agroindustrial use from primary forest soil of the Pungalá Parish, Riobamba Canton, and to analyze their physical-chemical properties, identifying the microorganisms through biochemical tests and the physical-chemical analysis of the soil through bromatological tests. The type of sampling carried out was random with the help of an auger, the sowing of the microorganisms in MRS agar, Acetobacter glucose and Saborau, being incubated for 48 hours at 37, 27 and the isolation was then carried out, and the identification of each microorganism was made by its morphology, color, surface, and Gram staining, and then proceeded with biochemical tests such as catalase, oxidase for lactic and acetic acid bacteria, and potassium hydroxide for lactic acid bacteria (LAB) and characterization tests such as carbohydrate fermentation and YPD broth in yeasts, obtaining 5 strains of lactic acid bacteria, 2 strains of (LAB) and yeasts, being all (LAB) positive for staining and negative for oxidase and catalase, as for KOH negative for two strains, In (BAL) all negative for staining and oxidase, but positive for catalase, for the physical chemical analysis was performed following the standards for each mineral, it was concluded that the soil is rich in minerals and the isolated strains belong to the genus lactobacillus, acetobacter and Saccharomyces, it is recommended biochemical tests such as API 50 CHL, or in a real-time PCR.