Estimation of IL-23 Among Patients with Vaginitis and Urinary Tract Infections
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Abstract
Background: E. faecalis must first avoid being eliminated by the host's clearance mechanisms. PMNs, or polymorph nuclear leukocytes, play an important role in the immune response of humans against bacterial infections.
Objective: Estimation of IL-23 among patients with vaginitis and urinary tract infections
Materials and methods: The study's 200 participants all provided urine and vaginal specimens from patients at Baghdad's Al-Karama Hospital and Medical City Hospital over the course of three months (May to July 2022), for (18-24) hours, the materials were grown in various media. The samples were then incubated at 37 degrees Celsius for 18 to 24 hours on various selective media. Colony morphology, microscopic inspections, and biochemical assays were used by scientists first to try and identify E. faecalis. Blood sample was collected from all 200 infected patients and 44 healthy patients as control. 5ml of fresh venous blood samples were putted in gel containing tubes was allowed to clot at room temperature for 30 minutes and then centrifuged at 3000×g for approximately 3 minutes.
Results: There were positive cultures obtained from all 200 clinical samples; however, only 44 (22%) of the isolates were linked to E. faecalis. The automated Vitek 2 system used GP-ID cards with 64 biochemical assays to confirm that the isolates were indeed E. faecalis. Fast identification of 44 distinct bacterial isolates was achieved using this method, with high confidence (probability percentages of (94 to 99.7%). In this study, determination of IL-23 in E. faecalis were investigated, and the results showed that, the highest mean of IL-23 level was found among patients with UTI infected with E. faecalis (287.54±12.18ng/ml) comparing with the control group (188.08±2.29ng/ml) with a significant difference between the two groups (P<0.05).
Conclusions: Identification of E. faecalis by using automated Vitek 2 system more specific than other biochemical tests. Interleukin-23 is elevated in E. faecalis infection.