Curcumin and nano-curcumin as inhibitors for carbonic anhydrase purified from human erythrocyte

Abstract

This study was aimed to inhibit carbonic anhydrase (CA) enzyme activity with curcumin (extract) and nano-curcumin (active compound) which had an antioxidant effect with low or no cytotoxic activity. Curcumin is natural and a bioactive compound found in the rhizome of turmeric (Curcuma longa L.), it can be used as a preventive and curative agent for many diseases such as diabetes, cancer, rheumatoid disease, allergy, and asthma and it has an effective role in eye diseases. Results of inhibition showed that nano-curcumin (active compound) was the most potent inhibitor than curcumin extract and the inhibitory percentage were 99 and 73.9 % respectively. Carbonic anhydrase enzyme purified from human erythrocytes by precipitation with 80% ammonium sulfate saturation, ion exchange chromatography and gel filtration chromatography using Sephadex G- 150 column. The specific activity of the crude enzyme was 9.1 U/mg protein reached 145 U/mg protein upon the last purification step. Characterization study of purified enzyme demonstrated that the optimum PH, PH stability, optimum temperature and temperature stability were 8, 8, 37 and (32-42 °C), respectively.