Transformation of Agrobacterium by cry1Ac gene from Bacillus thuringiensis

Abstract

Biotech crops express novel traits derived from other organisms, such as increased insect, herbicide, virus, and drought resistance. Among insect-resistant varieties, Bacillus thuringiensis (Bt) crops are most often used. Bt crops are engineered to produce Cry toxins from the soil bacterium Bt that compromise the gut integrity of members of specific insect families, making them highly selective insecticides. One such toxin is Cry1Ac (Cry), effective against the larvae of certain lepidopteran insects. Biotech crops express novel traits derived from other organisms, such as increased insect, herbicide, virus, and drought resistance. Among insect-resistant varieties, Bacillus thuringiensis (Bt) crops are most often used. Bt crops are engineered to produce Cry toxins from the soil bacterium Bt that compromise the gut integrity of members of specific insect families, making them highly selective insecticides. One such toxin is Cry1Ac (Cry), effective against the larvae of certain lepidopteran insects. The Cry1 gene (3537 bp) was loaded on a clone vector (PICS11052 T-DNA binary vector 4875 bp) to form a hybrid DNA. The hybrid DNA was introduced into Agrobacterium, the process of transformation into E. coli BL21(DE3), the bacteria were used to monitor the transformation process and the culture of the transformed bacteria on a medium containing the ampicillin.