Molecular detection of blaPER-1, blaVEB-1, and blaPSE-1 β-lactamase genes from P.aeruginosa Severe Urogenital UTI Infection

Abstract

Background: Nosocomial infections are mostly caused by Pseudomonas aeruginosa. Burn and wound infections are primarily caused by multidrug-resistant P. aeruginosa isolates. Nosocomial isolates often withstand several antibiotics, including new β-lactam antibiotics. Detecting multidrug-resistant strains might improve medication administration by (Davodian et al., 2015). Objectives: The objective of this study was to see whether P. aeruginosa isolates from people with urinary tract infections (UTIs) included the genes for class A ESBLs such PER-1, VEB-1, and PSE-1 by (Davodian et al., 2016). Methodology: In 2021 and 2022, 50 isolates were taken from the department of pathology's urine section at five different hospitals in Peshawar Pakistan. Clinical and Laboratory Standards Institute (CLSI) guidelines were used to do the antibiotic susceptibility test. Agar dilution method was used to find the MIC of ceftazidime by (Fernandes et al., 2013).  ESBL-producing isolates were looked for with the combine disc test. PCR (polymerase chain reaction) was used with specific primers to find the PER-1, VEB-1, and PSE-1 genes. Result: 60% (n=30) of the patients were male and 40% (n=20) were female. Most of the bacteria found in urine samples were resistant to the antibiotics augmentin (98.8%) and cefpodoxime (86%). Eighty percent of the isolates tested positive for ESBLs, with 94% (n=47) including the PER-1 gene, 55% (n=30) containing the VEB-1 gene, and 18% (n=10) containing the PSE-1 gene by (Fooladi et al., 2016). Conclusion: 10 isolates had blaPER1 and blaVEB1, and 7 amplified all three genes. ESBL-producing urine P. aeruginosa was common. BlaVEB1 and blaPER1 were common, but blaPSE1 was rare by (Fazeli, et al., 2015).