Toxoplasma gondii infection correlated to Interleukin 12 in wasit Women

Abstract

Background: Toxoplasmosis is a common and serious parasitic infection caused by the ubiquitous obligatory intracellular protozoan organism, Toxoplasma gondii. Moreover, it has been reported that IL-12 is important in initiating an effective cell -mediated immunity against  T. gondii tachyzoites,

Materials and Methods: Two hundred and seventeen blood specimens was collected from pregnant women with previous abortion, in addition to twenty specimens as a control from healthy women, through a skilled and professional procedure in a gel tube, then blood samples were centrifuged in which supernatant was extracted, extracted samples were preserved in refrigerator -4℃ until using later. Samples were taken from patients who were present in Al-Zahraa teaching hospital and Al-Kut teaching hospitals. Serological investigations were done to detect IgM and IgG in the sera of women using ELISA technique, in addition to detection of Interleukin 12 in aborted, pregnant and control samples.

Result:  Correlation  between IgM and IgG concentrations and Interleukin 12 outcomes indicated that Interleukin 12  was 4.13 pg/mL in cases of Toxoplasma  IgM and 4.12 pg/mL in Toxoplasma  IgG, regard CMV Interleukin 12 concentration was 3.79 pg/mL in cases of IgM and 3.67 pg/mL in IgG, about both infection cases results recorded 4.13 pg/mL and 4.43 pg/mL of 12 concentration in the cases of IgM and IgG respectively, finally control reported that 12 concentration  was 4.79 pg/mL in IgM cases and 3.93  pg/mL in IgG cases, (p value= 0.047). Regarding comparison between IL-12  concentration according to abortion, results showed that Toxoplasma  IL-12 concentration was 4.10 pg/mL in aborted and 4.01 pg/mL in non-aborted,  CMV IL-12 concentration was 3.77b pg/mL in aborted and 3.59 pg/mL in non-aborted, both IL-12 concentration was 4.09 pg/mL in aborted and 4.03 pg/mL in non-aborted state, (p value= 0.052).

Conclusion: Interleukin 12 was recorded as an important factor during Toxoplasma gondii infection.