Bacteriological study of Escherichia coli and its pathological role in urinary tract infection

Abstract

Background: One of the most prevalent bacterial infections in both adults and children around the world is urinary tract infections (UTIs). Bacteria cause more than (95%) of infections, including the majority of UTIs. The most frequent cause of urinary tract infections is Escherichia coli (E.coli).

Aims: to detect E.coli using culture media, as well as biochemical assays, and to analyze antibiotic resistance using the disc diffusion method, VITEK 2 system, and Biofilm from urine samples of patients with UTI.

Methods: A total of 471 midstream urine samples from UTI suspects were taken and tested for the presence of E.coli.

Results: The percentage of infection with E.coli was higher in females than in males, 39 (78%) and 11 (22%), respectively. The highest rate of bacterial infection was within the age group 21- 30 years and its rate was 51.28%, followed by the age group 41-50 years and its rate was 12.89% for females. All bacterial isolates were multi-drug resistant (MDR) used in the current study. The results showed that 48 (96%) bacterial isolates were highly resistant to Cefixime and Ceftriaxone, and also resistant to each of the ceftazidime (84%), followed by the two antibiotics, Tetracycline (72%) and Gentamicin (70%). The isolates showed a high ability to form biofilms.

Conclusion: The current investigation found that E.coli had developed resistance to numerous antibiotics.