Sequencing of Cytolethal Distending Toxin Gene among MDR E. coli isolates, Iraq
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Abstract
Background: Genome sequencing is a novel way to investigate an organism's genetic code.
Objective: E. coli isolates were sequenced for Cytolethal Distending Toxin gene
Materials and methods: 150 urinary tract infection patients at Maternity and Children Babylon Hospital were sampled. Healthy people provided 25 stool samples.
Results: Genetic analysis detected E. coli cdt genes in urine and stool samples. Five urine and five stool isolates were found in the gene bank. Sequences producing significant alignments of cdt gene with 100% similarity revealing the affinity of gene detection presented as cdt gene, however, high number of mutation were present in both stool and urine samples in general, the current study evaluated the effect of genetic viration on cdt-producing strains isolated from stool and urine samples and could further describe them.
Conclusions: The pathogenicity of E. coli was increased by presence of cdt gene.