Efficiency of Silver Nano Particles in Removing Pseudomonas Aeruginosa ATCC 27853 from Drinking Water Distribution Pipes

Abstract

Silver nanoparticles prepared by the simple green method using Azadirachta indica (A. indica) leaf extract. The formulation was found to be effective in terms of reaction time as well as stability of the synthesized nanoparticles with the exception of external stabilizing agents/reducing agents. It has been shown to be an environmentally friendly and rapid synthetic approach that provides a low-cost, high-efficiency method for the synthesis of silver nanoparticles; this reaction direction matches all conditions of the green chemical process and with a high percentage. In addition to the benefits of using plant extracts in synthesis, it saves energy, protects human health and the environment, and provides safer products, as the manufactured silver nanoparticles showed effective activity against Pseudomonas aeruginosa ATCC 27853. AgNPs was biologically (green method) prepared using leaf extract of neem plant (A. indica). Experimental lab unite was designed. Two PVC pipes (3 meters length, 2.54 cm diameter) were used, each pipe partitioned into six section of 50cm length, the pipes were alternately coated with silver nanoparticles(250ppm,125ppm). Both coated and uncoated pipe parts  were connected respectively to each other . Drinking water was artificially contaminated by 290 CFU Pseudomonas aeruginosa ATCC 27853 bacteria. The standard isolate of bacteria was obtained Pseudomonas aeruginosa ATCC 27853 used the (layered casting) method to find the effect of AgNPs prepared at different concentrations (125, 250) ppm and multiple periods (2, 5, 10, 15, 20) minutes. The result show that, the inhibition rate reached 100% at 250 ppm of AgNPs through the peiod of time15 and 20 minute,while with the concentration of AgNPs of 125 ppm, it did not achieve sufficient killing even after 20 minutes. UV-Vis spectrum, FTIR spectrum, XRD, FESEM and TEM, techniques were proved the success of the formation of silver nanoparticles. The pipes coated with silver nanoparticles were characterized using FE-SEM,TEM and XRD techniques.