Biodegradation of Crude Oil Contaminated Soil in North Oil Refineries/ Kirkuk /Iraq by Using Acinetobacter spp. and Streptomyces spp.

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Tawoos Mohammed Kamel Ahmed, Sameerah Faydhallah Mohammed, Khalid Mahmood Yosif

Abstract

The current work objective was to reveal the Biodegradation of the crude oil by Acinetobacter spp. and
Streptomyces spp. oil samples collected from tanks of north oil refineries/in in Kirkuk governorate/Iraq.
The current work was determining the crude oil biodegradation under various conditions. This work
revealed that led optimum conditions for the biodegrarocesses were pH seven even and temperature 37 0C.
Under this optimum condition, the rate of consumption reported, Acinetobacter spp. and Streptomyces spp.
The fourth day (pH 7: 28.3 and 28.1 and temperature 30 0C for Acinetobacter sp.: 38.4 and temperature 37
0C for Streptomyces spp. 27.4, respectively). Also, cell numbers reported Acinetobacter spp. and
Streptomyces spp. On the fourth day (pH 7: 11.1; 10.8 X log 10 and temperature 30 0C for Acinetobacter
spp.: 11.3 and temperature 37 0C for Streptomyces spp. 10.1 respectively). Also, consumption rate reported,
Acinetobacter spp. and Streptomyces spp. The Optimum incubation period (third day) is 35.9 and 30.1,
respectively, while cell numbers were reported for Acinetobacter spp. and Streptomyces spp. At optimum
incubation period (third day) is 11.2 X log 10 and 10.8 X log 10. It was concluded from this study that the
optimum conditions of Acinetobacter spp. and Streptomyces spp. to Biodegradation of crude oil were pH
7, temperature 37, and third incubation day.

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