Genetic diversity and relationship among (Vicia faba L.) Using SSCP –PCR- Sequencing Techniques

Abstract

Since their potential and genetic background were not thoroughly investigated, Algerian accessions constitute a crucial source of features of interest for crop enhancement, particularly for combating climate change. To evaluate the relationships and genetic diversity among 3 collected vicia faba isolated from various parts of Iraq, target region amplification polymorphism markers have been employed. For resolving genetic linkages across populations and to determine the genetic diversity of rare and endemic species, SSCP-PCR is helpful. Single-strand conformation polymorphism and polymerase chain reaction (PCR) have been utilized in order to genotype ITS4 gene. As a result, DNA sequencing was used for confirming such DNA polymorphisms. Depending on the SSCP-PCR method's results, there have been three distinct haplotype patterns present, with bands having bands of 4, 3, and 2 respectively. Yet, establishing the pattern regarding all resolved SSCP bands only through gel visualization might be challenging. After that, DNA sequencing should be used to confirm these DNA polymorphisms. According to the sequence's results, multiple SNPs were used to identify ITS region's haplotypes. All 3 sequenced isolates were deposited in GenBank at DNA Data Bank Japan (DDBJ) database with accession numbers (LC733679, LC733680, LC733681).

Conclusion: our research showed that the SSCP-PCR method is more useful for the assessment of the relation and genetic diversity among Vicia faba L populations.