Macroscopically and histopathological study of Using Fenestrated and Non-Fenestrated Catfish Acellular Dermal Matrix on Healing Ventro-Lateral Hernia in Bucks

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Fatima Amer Mohammad, Areege K Al-Ebadi

Abstract

Biological hernioplasty of the abdominal wall more benefit than prosthetic meshes. This study aimed to evaluate the decellularized fenestrated and non-fenestrated catfish (Arius bilineatus, Valenciennes) acellular dermal mateix in repaired ventro-lateral hernia in bucks. A 7 cm diameter right ventro-lateral hernia was induced under sedation and local anesthesia in 24 healthy male goats and left untreated for one month. The animals then were randomly divided into two groups with 12 bucks for each group and repaired by acellular catfish dermal matrix as fenestrated and non-fenestrated via on-lay technique and fixed by U-shape interrupted suture pattern. Macroscopic and microscopic assessments of graft ingrowth were performed at weeks 4, 8, and 12. The macroscopical examination revealed adhesions in both groups. The grade and severity of adhesion was more in fenestrated group in comparing with non-fenestrated group. Finally, both matrices were incorporated into the surrounding tissues in both groups. Histopathological examination indicated progression of healing process with few differences between groups, this represented by immature granulation tissue, a few inflammatory infiltration and numerous blood vessels with remnant of dermal matrices in non-fenestrated group at the three studied period.  In contrast dermal matrices in fenestrated group totally disappeared at 12 weeks post-treatment. At the end of experiment there were angiogenesis and well deposition of fibrous connective tissue which enable to close the abdominal wall defect in both groups. In conclusion, both types of catfish ADM may be serve as a good biocompatible biomaterial having good incorporation and enhancing the healing process in bucks.

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