Histopathologic changes in liver and kidney tissues induce by fungal toxins and treated by filtered fungi Agaricus bisporus and Sodium bicarbonate

Abstract

The study has the selection of   isolates of Aspergillus niger fungus and examining their ability to produce the Ochratoxin A and defining the toxic effects on some of the anatomic properties of the females of white rat. Moreover assessing the ability of filtered fungi Agaricus bisporus in reducing its toxicity, and when testing the ability of isolates of the fungus that is examined to produce the Ochratoxin A by using of the technology High Performance Liquid Chromatography (HPLC).The results of the histological study for the animal of the white rat that is treated with the Ochratoxin A where the tissue sections that have been taken from the liver shown the existence of pathological effects like a sever congestion in the tissues of the liver and loss of the radiative arrangement of the liver cells and an expansion in the liver pockets and breeding of the kupffer cells , while the kidney has been affected by the Ochratoxin A and the effect is represented in atrophy of the glomeruli and an expansion of the renal tubule twisted with (Degredation) and alienation in the cells of lining the renal tubules twisted Ochratoxin A with the existence of slight changes of the tissue in the organs of the rats treated with it. In the tissues of the liver and kidney, the results of test have shown and the laboratory diagnosis for the tissue sections for the mentioned organs that there is no occurrence of change of pathological tissue in the treatment of filtered fungi Agaricus bisporus and sodium bicarbonate and it is the symmetry of the tissue sections of the comparison treatment that referred to few pathological changes.

The effect of Ochratoxin A on levels of liver enzymes (ALT, AST, ALP) with levels of (44.57, 39.52 , 36.54) U/L respectively compared to control group (12.42 , 15.25 , 12.25)

U/L respectively The result of laboratory studay of the kidney function showed significant differences in the concentration of urea and creatinine rat tested (62.53) mg/dl and creatinine (1.852)mg/dl compared to control group (33.55)mg/dl and (0.621)mg/dl respectively. The result showed the effect of treatments Agaricus bisporus filtarate and sodium carbonate on liver and kidney tissues.