Isolation and Identification of Active Substances from Brown Algae and Study of their Biological Activity against Pathogenic Fungi

Abstract

The present study included the isolation and characterization of chemical compounds from the two alga Sargassum sp. and Laminaria sp. and the qualitative detection of the active compounds, the extracts of algae (hot water and ethanolic at a concentration of 80%) were banned using the Soxhlet device.

The primary detection showed the presence of secondary metabolites represented by phenols, flavonoids, tannins, glycosides, steroids and amino acids. The results of the gas chromatography-mass spectrometry detection revealed the presence of many active compounds, and the highest percentage among the active substances was for the compounds (n-Hexadecanoic acid and Oleic acid and Fucosterol) in both algae. The biological activity of the two algae extracts against fungi , Aspergillus niger , Candida albicans and Candida krusei was studied.

The hot aqueous extract of the two algae did not show any activity against the studied fungi, while the alcoholic extract of Sargassum algae showed biological activity as the inhibition diameters were (21.76, 22.00 and 20.76) against A.niger, C.albicans and C.krusei respectively, while The diameters of inhibition of the alcoholic extract of Laminaria moss were (20.90, 21.53, and 20.00) against A.niger, C.albicans , and C.krusei  respectively.