Synthesis and Spectroscopic Mixed Ligands Complexes of flavonoid and their Biological Study in Antitumor Activity for Treatment Breast Cancer Cell Line (MCF-7), Inhibitory Effect on Xanthine Oxidase and Antimicrobial

Abstract

The present study, the chalcone was prepared by Claisen-Schmidt condensation. Utilizing the spectrometers shown (FT-IR, UV-Vis, 1H-NMR, 13C-NMR, and mass spectra), the synthesized compound was then evaluated. A new  complexes was synthesized used the chalcone (HL1) as primary ligand and amino acid serine (HL4) as secondary ligand with [Co (II), Ni (II), Cu (II), Zn (II), and Cd (II)] ions. Additionally, using the spectroscopic techniques, all of the generated complexes have been identified (FT-IR, UV-Vis, 1H-NMR,       13C-NMR, magnetic susceptibility and conductivity studies). In the study findings indicated that all complexes had octahedral geometry and were nonconductive as measured by magnetic moments and molar conductance. The chalcone (HL1) and its copper complex were evaluated on the breast cancer cell line MCF-7 at dosages of (12.5, 25, 50, 100, 200) µg/ml, and the rate of cell growth inhibition was tracked for 48 hours of treatment. Comparing the effects of the copper complex and ligand on the MCF-7 breast cancer cell line before and after treatment, it was shown that the copper complex achieved good inhibition outcomes. The inhibition rate of ligand (HL1) and Cupper complex  between (1.66 – 71.00), IC50 = 47.98 µg/ml and IC50 =  28.09 µg/ml, respectively. The ability of chalcone (HL1) and its complexes with cobalt and copper to inhibit xanthine oxidase was studied. The inhibition was studied by monitoring the xanthine absorbance at the wavelength of 290 nm. The results showed good inhibition ratios, and that the copper complex gave the highest inhibition rates, with IC50 values of [Cu(L1)(L4)(H2O)2] = 2.36 *10-5 molar, [Cu(L1)(L4)(H2O)2] = 2.73 *10-5 molar, and HL1 = 4.79*10-5M molar. Three different types of bacteria were used to study the biological activity of the synthesized ligands and their complexes with concentration (1×10-2, 1×10-3, 1×10-4) molar utilizing the inhibition approach (Staphylococcus aureusa, Escherichia coli and Burkholderia cepacia). To measure its potential antibacterial effect, this was done. The experiments generally indicated that the ligands and their complexes have modest or moderate action against the different kinds of bacteria and  as follow:

Staphylococcus aureus > Escherichia coli > Burkholderia cepacia