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Background: Preserving cadavers is crucial for anatomical studies, but traditional methods using chemicals
such as formalin can have harmful properties. Ayurveda, like other sciences, lacks an alternative method for
preservation. This study aimed to find a safe and effective herbal preservative for cadavers.
Aim: The aim of this study was to formulate an herbal preservative solution for human cadavers that ensures
no risk of infection on contact, preservation of the body, and prevention of putrefaction changes and
contamination with maggots and insects.
Objective: The objectives of the study were to identify natural preservative ingredients used in Ayurveda,
research individual ingredients for their possible role as preservatives, and prepare and test an herbal solution
on chicken muscle pieces.
Material and Methods: A detailed literature survey was conducted to identify natural preservative ingredients
used in Ayurveda. The individual ingredients were researched for their possible role as preservatives. An herbal
solution was prepared using the identified ingredients, and its efficacy was tested on chicken muscle pieces.
Results: The study identified natural preservative ingredients such as Amalaka, Vibhitaki, Haritaki, Neemba
and Vidanga. These ingredients were found to have antimicrobial, antioxidant, and anti-inflammatory
properties that could potentially preserve human cadavers. Tested herbal preservative solution on chicken
muscle pieces at different concentrations. Preservation duration increased with higher concentration of
solution. Maximum preservation period was achieved with 100% solution with no signs of contamination with
maggots and insects.
Conclusion: This study provides a potential alternative to traditional chemical embalming methods and may
enable Ayurvedic graduates to successfully practice medicine and surgery. To validate the effectiveness of the
herbal solution on human cadavers, additional research is required. Moreover, in the main study, it is
recommended to substitute certain drugs that could potentially enhance tissue preservation longevity in this