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The acute toxicity, median lethal concentration and safe concentration of Terramycin 200 in an Indian snakeheaded Murrel, Channa punctatus (Bloch) of body weight 40-50g were evaluated applying various methods.
Lorke and Enegideet al.,estimated the 96hr-LC50 value to be 2711.09 and 2800.0 mg/L. The Up-and-Down
method yielded a 96hr-LC50 range of 2216.67 to 2683.33mg/L as a rough estimate. Furthermore, the BehrenKarber, Miller and Tainter and Finney methods had 96hr-LC50 values of 2450.0, 2390.0, and 2511.88mg/L,
respectively. Finally, the Reed-Muench method was used to calculate the 96hr-LC50 dose as well as the
median ideal dose of 3129.68mg/L. The cumulative median lethal average of 96hr-LC50 dose in Channa
punctatus was 2450.0+2390.0+2511.88+3129.68 = 2620.39 mg/L of Terramycin 200. Among the various
methods used, the Chinedu method was found to be the most accurate. There was a significant relationship
between the dose of Terramycin 200 and fish mortality. Terramycin 200 accumulates in aquatic bodies,
disrupting the physiological activities of this fish, according to the findings. As a result, caution should be
exercised when using high concentrations of Terramycin to treat Channa punctatus.
It can be deduced that this fish is more resistant to Terramycin 200 toxicity than other fishes. The calculated
safety level value indicated that determining an acceptable dose of Terramycin 200 for this fish is feasible.
Terramycin 200 may even be classified as a slightly/moderately cytotoxic substance to Channa punctatus
based on its toxicity range.