Formulation And Evaluation Of Antimicrobial Gel Of Clitoria Ternatea(Flowers)

Abstract

To formulate novel topical herbal gel prepared by Clitoria ternatea flowers and evaluate their antimicrobial activity. Dried, powdered flowers were extracted with ethanol using maceration method for 24 hrs. Drug-excipients compatibility study were performed for the selection of formulation excipients. Topical formulations like gel containing C. ternatea extract were formulated using combination of gelling agents such as HPMC K100M and Carbopol 934 P. Prepared gels were subjected to various evaluation parameters such as physicochemical parameters, pH, viscosity, spreadability, homogeneity, drug content uniformity, in-vitro drug diffusion, permeability and antimicrobial activity. A topical gel was successfully formulated containing bioactive ethanolic extract of C. ternatea flowers. The gel was found to be very effective as antimicrobial formulations. Prepared topical gel of Clitoria ternatea was shown pH range 6.2 to 6.6, viscosity range 480 to 603cp, spreadability range 20.62 to 24.33g.cm/s and homogeneous. Prepared gel was shown uniformity in
drug content which ranges from 94.32 to 96.62%, zone of inhibition ranges 12mm for Bacillus spp. Prepared gel formulations were shown drug release, not more than 85% in 6 hr. As the concentration of Carbopol 934 P and HPMC K100M was increased from 0.5 to 2.0gm and 5.0 to 6.5gm respectively in the gel, antibacterial activity was synergistically improved against Bacillus spp.