Improvment Of Phaseolus Vulgaris Seed Germination With GA3 Under Salt Stress

Abstract

Salinity is one of the main abiotic stresses that causes a serious damage to agricultural production. This study aimed to investigate and evaluate the impact of increasing salt stress levels on seeds germination of bean species (Phaseolus vulgaris L.) This research is based on biochemical and physiological measurements. To select salt-tolerant genotypes and propose a biological alternative, the effect of different concentrations of  NaCl (50, 100, 150 and 200 mM) and an exogenous application of GA₃ on the seeds were evaluated. Generally, GA₃ is a  hormone that improves plant growth. The negative effect of salt stress induced by NaCl was observed on the percentage of imbibition, the radicles length. under this stress, an increase in soluble sugar amount was recorded compared to the control (0 mM). Indeed, the high value of soluble sugars was recorded by Cocorose variety. In this study, the α-amylases activity after 72 hours seems sensitive to salinity. Consequently, 0.759 mg of maltose are obtained by Coco rose genotype, While, Djadida genotype registred 0.516 mg. At 72 hours, this activity seems lower, Coco rose genotype showed 0.136mg in 0mM. The application of GA₃ (10^-5 mM) exerted a notable effect on the increase in the activity of α-amylases.

The results proposed that GA₃ can be used as an alternative to improve the physiological and biochemical response of plants under abiotic stress.