Prevalence Of Alp And Asp Genes In Aspergillus Fumigatus Isolated From Iraqi Patients

Abstract

Background: Pulmonary aspergillosis is a severe infection in immunocompromised caused by Aspergillus fungi, with
Aspergillus fumigatus being the most frequently cultured of this genus. Fungal proteases such as serine and a aspartate
proteases have diverse importance in fungal pathogenicity.
Materials and Methods: A total of 105 samples were collected from TB patients with symptom of lung infection . These
sputum samples were obtained from patients treated in Public health department Chest and Respiratory diseases clinic ,
from February 2023 to April 2023. All samples information were recorded including age, sex and related diseases, after
that PCR was done to detect alp and asp using specific primers.
Result: Association between culture results of A.fumigatus and patient parameters was done using patients parameters
included gender, age, tuberculosis bacilli (T.B) history, diabetes, hypertension and residency, gender were divided into
male and female, outcomes were 9 (8.5%) and 5 (4.8%), age groups number and percentage were 0(0.0%), 3(2.9%),
1(0.9%), 2(1.9%), 3(2.0%) and 5(4.7%) in the age groups <=29, 30-39, 40-49, 50-59, 60-69 and >=70 of A.fumigatus
regarding total number, T.B history, diabetes, hypertension and residency were related to 14 (100%) of A.fumigatus, T.B
history was recorded in 1(17.1%) of patients, diabetes number and percentage were 7(50.0%), hypertension 8(57.1%),
residency was divided into urban 9(64.3%) rural 5(35.7%). Additionally estimation prevalence of alp and asp genes in
A.fumigatus fungal isolates has been showed highly prevalence in isolates.
Conclusion: Our data showed increase prevalence of alp and asp genes in Aspergillus fumigatus in Iraqi patients. This
prevalence should be alarming public health.