INCREDIBLE RESEARCH WITH MURASHIGE AND SKOOG MEDIUM (MS) IN PLANT TISSUE CULTURE ON SELAGINELLA BRYOPTERIS (SANJEEVANI BOOTI)

Abstract

Medicinal plants have been utilized as therapeutic resources to address various human health disorders from ancient times to the present. They constitute a significant natural wealth, providing essential medical care to people across different walks of life. These plants serve not only as vital therapeutic agents but also as key raw materials for the production of both traditional and modern medicines. Various parts of medicinal plants, including seeds, flowers, roots, leaves, fruits, peels, and even entire plants, are used for medicinal purposes. These plants are rich in metabolites with remarkable properties, such as carbohydrates, tannins, flavonoids, alkaloids, terpenoids, and steroids, which are effective in treating numerous diseases.

With advancements in modern techniques, several specific protocols have been developed for the commercial-scale production of a wide range of secondary plant metabolites. Plant tissue culture has recently made significant contributions and now stands as an indispensable tool for the progress of agricultural science and modern agriculture. Various treatments can induce shoot and leaf development, with the most effective being the application of 1.5 mg/L BAP. In vitro-raised Selaginella bryopteris were planted in pots and grown for approximately 2-3 months in polyhouse conditions for further study. This research aims to analyze the advancements in plant tissue culture for agriculture, contributing to human health and well-being.