Acute Toxic Study And Pharmacogenetic Analysis Of The Methanolic Extract Of Justicia Adhatoda

Abstract

Acute oral toxicity studies aim to determine the therapeutic index of a substance. A pharmacogenetic study was conducted on the methanolic extract of Justicia adhatoda L, following the revised draft guidelines 423 of the Organization for Economic Co-operation and Development (OECD). The study involved methods such as color and odor, ash value, extractive value, and limit test for heavy metals. The results showed that the alcoholic extract or aqueous extract suspension was orally fed to overnight starved wistar albino rats at a dose of 2000mg/kg body weight. Behavioral changes were observed at intervals of 4 hours, 6 hours, 24 hours, and 48 hours during the next 14 days.

The study used healthy young albino rats, both male and female, weighing between 150-200 g and aged 8 to 12 weeks. The LD50 (lethal dose 50%) of an extract from a specific medicinal plant called Adhatoda Vasika was determined. The animals were initially monitored after receiving a dose, at least once during the first 30 minutes and then periodically throughout the first 24 hours. Mortality was recorded in all instances within the initial 24-hour period. Further attention was devoted to the examination of convulsions and tremors.