Anaesthetic effect of Sodium-Thiopental by bath on the three different body weights of Rainbow trout (Oncorhynchus mykiss, Walbaum, 1792)

Abstract

The aim of this study was the efficacy of sodium-thiopental as an aneasthetic by bath on three different body weights of rainbow trout (Oncorhynchus mykiss). During the experiment, aeration had done and physicochemical properties of water such as dissolved O₂, temperature and pH were recorded. Trouts were sorted into three groups with different mean body weights of 8.31±0.36g, 22.58±0.61g and 81.61±1.76g.  Every three groups were anaesthetized by bath, with 100 mg/L (100 ppm) of sodium-thiopental concentration. The results showed that the induction and recovery times, being significantly affected by sodium-thiopental, so that, these were shorter in the heavier fish. Anaesthesia duration for the fish with different mean body weights of 8.31±0.36g, 22.58±0.61g and 81.61±1.76g were thiopental with the weight of fish. So that the elapsed and recovery times were shorter in more weight fish than lighter ones.271±64.88, 240±3.33 and 231±76.47 seconds respectively. Also, recovery times were 448±67.81, 415±10.80 and 383±57.55 seconds respectively. Monitoring for 2 hours was shown that all fish have been alive. So, 100mg/L concentration of sodium thiopental is safe in the trout. The results of the present study showed that there was an inverse relationship between the elapsed and recovery times of sodium.