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Laundry waste is a biodegradable material that very suitable to use to biological processes. Downflow Hanging Sponge (DHS) is one of them. DHS is a biological treatment with an attached growth method. DHS uses a sponge medium that provides a three-dimensional space in which microorganisms can grow. The purpose of this study was to determine the effectiveness of laundry waste treatment in reducing pollutant levels using DHS so that it meets the quality standards. The variables used in this study are media, debit, and Hydraulic Retention Time (HRT). The results of the research showed that cellulose sponge media with 3 hours of HRT was the most optimum in reducing COD in the range of 62- 90%, BOD in the range of 56-82%, TSS in the range of 80-92%, Phosphate in the range of 21-72%, and also MBAS with a range of 27-76%.