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In recent times, green synthesis of nanoparticles had received significant consideration due to the requirement of non-hazardous chemicals, low-cost approaches, renewable materials and eco-friendly alternative to the traditional production processes because of its growing industrial applications. The present study focused on synthesis of Spirulina mediated Titanium dioxide nanoparticles (TiO2 NPs) was used to study its effects on economic traits of silkworm (Bombyx mori L.). The green synthesis of TiO2 NPs was characterized by UV, PSA, XRD, FTIR, SEM and TEM. In this study the UV-Visible absorbance spectroscopy result was found to be nanoparticles were formed at the range of 300 nm. SEM images shows that the nanoparticles are in spherical shape and TEM images shows that the nanoparticles are spherical in shape and their size range from 50 - 60 nm.