Human Parvovirus B19 Detection In Thalassemic Patients Associated Serological Responses In Al Najaf Governorate

Abstract

This study was conducted at from Al zahraa teaching hospital in alnajaf city between August (2022) and
January (2023). This study is aimed to detect the prevalence of the Parvovirus B19 on patients with
Thalassemia and study the immunological responses of cytokines, IL-6 and C-RP . This study included 105
samples from both genders and different age categories, the samples were divided into two groups: the first
group (study group) contained the Thalassemia which included 70 samples and the second group contained
35 control specimens (healthy group). Blood samples for study groups were collected from the patients of the
blood diseases center and the Elisa was used to detect the Viral (IgG) and Afias-6 was used to measure the
concentrations of IL-6 and C-RP in sera patients. The current study showed that viral infection with
thalassemia patients was 58.6% while no viral infections in the control group with a high significance
difference of P.v= 0.002. The results also revealed that the viral infection was higher in males than in
females with 35.7% and 22.9% respectively with presented high significance difference P.v= 0.005 .The
results referred that the highest infection rate in regards to age categories was in age category of 16-26 years
old with infection rate of 41.4% and the least was the age category of more than 49 years old with percentage
of 0 % with present of significance difference P.v= 0.005 .
The results referred that viral infection rate was a high percentage in the blood transfusion recipients of one
blood transfusion routine each 20 days followed by the recipients of one blood transfusion routine every 30
days and lastly the recipients of one blood transfusion routine every 40 days with infection rate of (52.1% ,
55% and 50%)
respectively, with presented a high significance difference P. v = 0.006
The results of IL-6 showed presence of high significant difference between patient and control. The
mean and standard deviation of IL-6 for patient was 11.78±1.1 pg/ml while for control was 5.29±0.36
pg/ml. and The results of C-RP showed presence of high significant difference between patient and
control . The mean and standard deviation of C-RP for patient was 12.7±1.71 mg/dl while for control was
4.61±0.32 mg/dl.